Resources

Scientific Evidence Behind the HPAE Platform

A central reference library for academic researchers, licensing partners, investor due-diligence teams and industrial clients.

Publications

Selected Peer-reviewed publications

Foundational HPAE synthesis and gene delivery

Cutlar, L., Zhou, D., Gao, Y., Zhao, Y., Greiser, U., Wang, W. & Wang, W. (2015). Highly Branched Poly(beta-Amino Esters): Synthesis and Application in Gene Delivery. Biomacromolecules, 16(9), 2609-2617.

DOI: https://doi.org/10.1021/acs.biomac.5b00966

Landmark demonstration that branching matters

Zhou, D., Cutlar, L., Gao, Y., et al. (2016). The Transition from Linear to Highly Branched Poly(beta-amino ester)s: Branching Matters for Gene Delivery. Science Advances, 2(6), e1600102.

DOI: https://doi.org/10.1126/sciadv.1600102

Early therapeutic application in skin gene therapy

Zhou, D., Gao, Y., Aied, A., et al. (2016). Highly Branched Poly(beta-amino ester)s for Skin Gene Therapy. Journal of Controlled Release, 244, 336-346.

DOI: https://doi.org/10.1016/j.jconrel.2016.06.014

Delivery of minicircle DNA

Liu, S., Gao, Y., Zhou, D., et al. (2019). Highly Branched Poly(beta-amino ester) Delivery of Minicircle DNA for Transfection of Neurodegenerative Disease-Related Cells. Nature Communications, 10, 3307.

DOI: https://doi.org/10.1038/s41467-019-11190-0

HPAE technology for hereditary skin diseases

Zeng, M., Xu, Q., Zhou, D., et al. (2021). Highly Branched Poly(beta-amino ester)s for Gene Delivery in Hereditary Skin Diseases. Advanced Drug Delivery Reviews, 176, 113842.

DOI: https://doi.org/10.1016/j.addr.2021.113842

Advanced three-dimensional HPAE architecture

Li, Y., Wang, X., He, Z., et al. (2023). 3D Macrocyclic Structure Boosted Gene Delivery: Multi-cyclic Poly(beta-Amino Ester)s from Step Growth Polymerization. Journal of the American Chemical Society, 145, 17187.

DOI: https://doi.org/10.1021/jacs.3c04191

HPAE-enabled topical CRISPR therapy for RDEB

Wang, X., Wang, X., Li, Y., et al. (2023). CRISPR/Cas9-Based Non-Viral Gene Editing Therapy for Topical Treatment of Recessive Dystrophic Epidermolysis Bullosa. Molecular Therapy - Methods & Clinical Development, 31, 101134.

DOI: https://doi.org/10.1016/j.omtm.2023.101134

Backbone optimisation for enhanced delivery

Li, Y., Qiu, B., Li, Z., et al. (2024). Backbone Cationized Highly Branched Poly(beta-Amino Ester)s as Enhanced Delivery Vectors in Non-Viral Gene Therapy. Journal of Controlled Release, 367, 327-338.

DOI: https://doi.org/10.1016/j.jconrel.2024.01.046

Delivery of dual CRISPR ribonucleoproteins

Wang, X., Li, Y., Friess, D., et al. (2025). Guanidyl-Rich Highly Branched Poly(beta-amino ester)s for the Delivery of Dual CRISPR Ribonucleoprotein for Efficient Large DNA Fragment Deletion. Journal of Controlled Release, 379, 549-557.

DOI: https://doi.org/10.1016/j.jconrel.2025.01.032

Application to lentiviral vector production

Wei, M., Yao, L., Wang, X., et al. (2026). Concentration of DNA at the Cell Surface Dictates Transfection Efficacy: A Hyperbranched Poly(beta-Amino Ester)-Mediated Strategy for Enhanced Lentivirus Production. Polymers, 18(9), 1015.

DOI: https://doi.org/10.3390/polym18091015